Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA
DOI:
https://doi.org/10.29244/jtcs.4.3.102-107Abstract
Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.
Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.
References
Al-Saif, A.M., Sharif Hossain, A.B.M., and Taha, R. M. (2011). Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro. African Journal of Biotechnology 10, 5291-5295.
Badan Pusat Statistik [BPS]. (2014). Basis Data Statistik Pertanian http://aplikasi.pertanian.go.id/bdsp/newkom.asp. [June 12, 2015]
Dhekney, S.A. (2004). “Molecular Investigations, Cryopreservation and Genetic Transformation of Papaya”. Thesis. University of Florida, Florida, USA.
Dhekney, S.A. Kandel, R., Bergey D.R., Sitther V., Soorianathasundaram, K., and Litz R.E. (2016). Advances in papaya biotechnology. Biocatalysis and Agricultural Biotechnology 5, 133–142,
Drew, R.A, McComb J.A., and Considine,J.A. (1993). Rhizogenesis and root growth of Carica papaya L. in vitro in relation to auxin sensitive phase and use of riboflavin. PIant Cell Tissue and Organ Culture 33, 1-7.
Drew, R.A. (1992). Improved techniques for in vitro propagation and germplasm storage of papaya. HortScience 27, 1122 -1124.
Drew, R.A. (1988). Rapid clonal propagation of papaya in vitro from mature field grown trees. HortScience 23, 609-611
Drew, R.A. and Miller, R.M. (1984). Nutritional and cultural factors affecting rooting of papaya (Carica papaya L.) in-vitro. HortScience 64, 767-773.
Drew, R.A. and Smith, N.G. (1986). Growth of apical and lateral buds of pawpaw (Carica papaya L.) as affected by nutritional and hormonal factors. HortScience 61,535-543.
Fitch, M.M., Terry, L., Saito, N., Yamamoto, G. Dela Cruz, A., Yeh, Aileen, W., White, S., Maeda, S.H., Ferreira, S., and Moore, P. (2003). Photoautotrophic rooting and growth of papayas in vitro Abs. 572. Annual Meeting of American Society of Plant Biologists Plant Biology p. 131, July, 25–30, Honolulu, Hawaii.
Keng C.L. and Teo K.H.C. (1994). Culture of papaya explant in solid-liquid media sequence as a rapid method for producing multiple shoots. Journal of Tropical Agricultural Science 17, 103-106.
Lai, C.C., Yu, T.A., Yeh, S.D., and Yang, J.S. (1998). Enhancement of in vitro growth of papaya multishoots by aeration. PIant, Cell, Tissue and Organ Culture 53, 221-225.
Minocha, S.C. (1987). Plant growth regulators and morphogenesis in cell and tissue culture in forest trees In Cell and Tissue Culture in Forestry Vol. I. (J.M. Bonga and D.J. Durzan, eds) p.50 – 66. Martinus Nijhoff Publishers.
Mumo, N.N., Rimberia, F.K., Mamati, G.E., and Kihurani, A.W. (2013). In vitro regeneration of selected Kenyan papaya (Carica papaya L.) lines through shoot tip culture. African Journal of Biotechnology 12, 6826-6832.
Ncube, T.N., Greiner, T., Malaba, L.C., and Gebre-Medhin, M. (2001). Supplementing lactating women with pureed papaya and grated carrots improved vitamin A status in a placebo-controlled trial. The Journal of Nutrition 131, 1497-1502.
Paulos, M., Joshi, V.R., and Pawar, S.V. (2013). Effect of BAP and NAA on in vitro shoot establishment and proliferation of banana (Musa paradisiaca) Cv.Grand Naine.
International Journal of Science and Research 4, 310-323.
Saksena, P. and Sharma, N.C. (2014). In vitro propagation in four dioecious varieties of papaya (Carica papaya). World Journal of Applied Science Research 4, 24-31.
Singh, B., Cheek, H.D., and Haigler, C.H. (2009). A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber. Plant Cell Report 28,1023-1032. doi: 10.1007/s00299-009-0714-2.
Sujiprihati, S., Gunawan, E., Darma, K., Kurniawan, A., Hidayat, and Gunarso (2010).
“Deskripsi Papaya Varietas “Callina”. Center for Tropical Horticulture Studies. Bogor Agricultural University, Indonesia.
Weaver, J.R. (1972). Plant Growth Substances in Agriculture. University of California, Davis WH. Freeman and Co. San Fransisco.
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