The Potentials of Limau (Citrus amblycarpa Hassk. Ochse) as A Functional Food and Ornamental Mini Tree Based on Metabolomic and Morphological Approaches

Authors

  • Rahmat Budiarto Bogor Agricultural University
  • Roedhy Poerwanto Bogor Agricultural University
  • Edi Santosa Bogor Agricultural University
  • Darda Efendi Bogor Agricultural University

DOI:

https://doi.org/10.29244/jtcs.4.2.49-57

Abstract

Limau (Citrus amblycarpa (Hassk.) Ochse) is one of the native Citrus species of West Java that is grown in the local backyard, thus is easily found in local cuisines. Limau has great potentials for use as a potted plant in home gardens for its aesthetic value and fragrance. The objective of this research was to describe the metabolites fingerprint and morphology of Limau as a potential functional and ornamental mini tree. Methanol leaf extracts from one-year-old Limau originated from Bogor were analyzed using untargeted Gas Chromatography Mass Spectrometry (GCMS). Methanol leaf extract was dominated by vitamin E and composed of other 18 metabolites including phytosterols, fatty acids and terpenes. The major portion of fruit extract consisted of 6-octadecenoic acid, palmitate and various fragrance compounds such as alpha sinensal, alpha limonene, beta citronellal, citronellol, and sabinene. Limau fruits are also a functional food due to its rich antioxidant and aromatic content in the fruits. The morphology of limau tree is suitable as a potted ornamentals or mini-fruits tree for its small crown, sparse branching with unifoliate aromatic leaves and small spines, and plentiful aromatics mini fruits with the diameter of around 2-4 cm. 

 

Author Biographies

Rahmat Budiarto, Bogor Agricultural University

Graduate student in Department of Agronomy and Horticulture

Roedhy Poerwanto, Bogor Agricultural University

Professor in Department of Agronomy and Horticulture

Edi Santosa, Bogor Agricultural University

Lecture in Department of Agronomy and Horticulture

Darda Efendi, Bogor Agricultural University

Lecture in Department of Agronomy and Horticulture

References

BMKG. (2017). Data Online Iklim BMKG Periode Januari-April 2017. http://bmkg.go.id. [10 May, 2017]

Bradford, P.G. and Awad, A.B. (2007). Phytosterols as anticancer compounds. Molecular Nutrition and Food Research 51, 161-167.

Budiyati, E. (2014). Keragaman plasma nutfah jeruk dan pengembangannya sebagai substitusi buah impor. http://balitjestro.litbang.pertanian.go.id/keragaman-plasmanutfah-jeruk-dan-pengembangannya-sebagai-subtitusi-buah-impor/ [10 May, 2017]

Burton, G.W. and Traber, M.G. (1990). Vitamin E: antioxidant activity, biokinetics, and bioavailability. Annual Review of Nutrition 10, 357-82.

Chempages. (2017). Gas Chromatography: Calculating the Area. http://faculty.sdmiramar.edu/ fgarces/labmatters/chemtech/modules/gaschrom/gaschromcalc.html [20 May, 2017].

Choi, M.Y., Choi, W., Park, J.H., Lim, J., and Kwon, S.W. (2010). Determination of coffee origins by integrated metabolomic approach of combining multiple analytical data. Food Chemistry 121, 1260-1268.

Clish, C.B. (2015) Metabolomics: an emerging but powerful tool for precision medicine. Cold Spring Harbor Molecular Case Studies 1, 1-6.

Darmawan, M., Poerwanto, R. and Susanto, S. (2014). The application of prohexadion-ca, paclobutrazol, and strangulation for off season flowering induction of mandarin citrus plants (Citrus reticulata). Journal of Horticulture 24, 133-140.

Del Bosco, S.F. (2003). The use for ornamental purposes of an ancient citrus genotype. Acta Horticulturae 598, 65–67.

Dorji, K. and Yapwattanaphun, C. (2011). Morphological identification of mandarin (Citrus reticulata Blanco) in Bhutan. Kasetsart Journal Natural Science 45, 793–802.

Dos Santos, A.R.A., De Souza, E.H., Souza, F.V.D., Fadini, M., Girardi, E.A. and Filho, W.D.S.S. (2015). Genetic variation of Citrus and related genera with ornamental potential. Euphytica. doi 10.1007/s10681-015-1423-2

Dirjenhort. (2016). “Petunjuk Umum Program Peningkatan Produksi dan Nilai Tambah Produk Hortikultura Tahun 2017”. Direktorat Jenderal Hortikultura (Dirjenhort) Kementerian Pertanian, Indonesia.

Duetz, W.A., Bouwmeester, H., Beilen, J.B., and Witholt, B. (2003). Biotransformation of limonene by bacteria, fungi, yeasts and plants. Applied Microbiology and Biotechnology 61, 269-277.

FAO. (2016). “Citrus Fruit Statistics 2015”. Food and Agriculture Organization (FAO) of The United Nations, Rome.

Hall, R.D. (2006). Plant metabolomics: from holistic hope, to hype, to hot topic. New Phytologist 169, 453-468.

HMDB. (2017a). Alpha-sinensal. http://www.hmdb.ca/metabolites/HMDB0013693 [May 5, 2017].

HMDB. (2017b). Alpha-farnesene. http://www.hmdb.ca/metabolites/HMDB0036066 [May 5, 2017].

Huang, C.B., Alimova, Y., Myers, T.M., and Ebersole, J.L. (2011). Short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms. Archives of Oral Biology 56, 650-654.

IPGRI. (1999). “Descriptors for Citrus”. International Plant Genetic Resources Institute (IPGRI), Rome.

Irsyam, A.S.D. (2015). “Floristic Study on Rutaceae of Madura”. Thesis. Bogor Agricultural University.

Janssen, A.M., Sioe, W.H.R.T., Scheffer, J.J.C. and Svendsen, A.B. (1998). Citronellal and citronellol, a case of antimicrobial antagonism. Flavour and Fragrance Journal 3, 137-140.

Jones, P.J. and AbuMweis, S.S. (2009). Phytosterols as functional food ingredients: linkages to cardiovascular disease and cancer. Current Opinion Clinical Nutrition and Metabolic Care 12, 147-151.

Jumhawan, U., Putri, S.P., Yusianto, Marwanni, E., Bamba, T., and Fukusaki, E. (2013). Selection of discriminant marker for authentication of Asian palm civer coffee (kopi luwak): a metabolomics approach. Journal of Agriculture Food Chemistry 61, 7994-8001.

Kariyat, R.R., Hardison, S.B., de Moraes, C.M., and Mescher, M.C. (2017). Plant spines deter herbivory by restricting caterpillar movement. Biology Letters 13, 1-5.

Kelly, G.S. (1999). Squalene and its potential clinical uses. Alternative Medicine Review 4, 29-36.

Kosmides, A.K., Kamisoglu, K., Calvano, S.E, Corbett, S.A. and Androulakis, I.P. (2013). Metabolomic fingerprinting: challenges and opportunities. Critical Review in Biomedical Engineering 41, 205–221.

Kumar, S.S., Samydurai, P., Ramakrishnan, R., and Nagarajan, N. (2014). Gas chromatography and mass spectrometry analysis of bioactive constituents of Adiantum capillus-veneris L. International Journal of Pharmacy and Pharmaceutical Sciences 6, 60-63.

Labkesda. (2017). “Prosedur Analisis GCMS Tanaman”. Laboratorium Kesehatan Daerah Provinsi DKI Jakarta (Labkesda), Jakarta.

Lim, T.K. (2012). “Edible Medicinal and Non Medicinal Plants: Volume 4 Fruits”. Springer, Berlin.

Lv, X., Zhao, S., Ning, Z., Zeng, H., Shu, Y., Tao, O., Xiao, C., Lu, C., and Liu, Y. (2015). Citrus fruits as a treasure trove of active natural metabolites that potentially provide benefits for human health. Chemistry Central Journal 9, 1-14.

Nakai, S., Yamada, S., and Hosomi, M. (2005). Anti-cyanobacterial fatty acids released from Myriophyllum spicatum. Hydrobiologia 543, 71-78.

NOPD. (2017). Terminology. http://www.northernontarioflora.ca/ [May 1, 2017].

Okoye, N.N., Ajaghaku, D.L., Okeke, H.N., Ilodigwe, E.E., Nworu, C.S., and Okoye, F.B. (2014) Beta-amyrin and alpha-amyrin acetate isolated from the stem bark of Alstonia boonei display profound anti-inflammatory activity. Pharmaceutical Biology 52, 1478-1486.

Othman, S.N.A.M., Hassan, M.A., Nahar, L.., Bazar, N., Jamil, S., and Sarker, S.D. (2016). Essential oils from the Malaysian Citrus (Rutaceae) medicinal plants. Medicines 3, 1-11.

Ovesna, Z., Vachalkova, A., and Horvathova, K. (2004). Taraxasterol and beta-sitosterol: new naturally compounds with chemoprotective/chemopreventive effects, Minireview. Neoplasma 51, 407-414.

Pennisi, B.V. (2017). “Gardening in Containers using Tropical Plants”. UGA Cooperative Extension, University of Georgia, Athens.

Placek, L.L. (1963). A review of petroselenic acid and its derivatives. Journal of American Oil Chemists’ Society. 40, 319-329.

Poerwanto, R. and Susila, A.D. (2014). “Teknologi Hortikultura”, IPB Press, Bogor.

Prabhadevi, V., Sathish, S.S., Johnson, M., Venkatramani, B., and Janakiraman, N. (2012). Phytochemical studies on Allamanda cathartica L. using GC-MS. Asian Pacific Journal of Tropical Biomedicine, s550-s5554.

Pubchem. (2017). Methyl oleate. https://pubchem.ncbi.nlm.nih.gov/compound /methyloleate. [May 5, 2017].

Pusdatin. (2015). “Outlook Komoditas Pertanian Subsektor Hortikultura : Jeruk” (Nuryati, L., Novianti, eds.). 88 p. Pusat Data dan Sistem Informasi (Pertanian) Kementrian Pertanian, Jakarta.

Putri, S.P., Nakayama, Y., Matsuda, F., Uchikata, T., Kobayashi, S., Matsubara, A., and Fukusaki, E. (2013a). Current metabolomics: practical applications. Journal of Bioscience and Bioengineering 115, 579-589.

Putri, S.P., Yamamoto, S., Tsugawa, H., and Fukusaki, E. (2013b). Current metabolomics: technological advances. Journal of Bioscience and Bioengineering 116, 9-16.

Raman, V.B., Samuel, L.A., Saradhi, M.P., Rao, B.N., Khrishna, A.N.V., Sudhakar, M., and Radhakhrishan, T.M. (2012). Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian Journal of Pharmaceutical and Clinical Research 5, 99-106.

Ramsden, J.J. (2009). “Bioinformatics, An Introduction”. 272 pp. Springer-Verlag, London.

Sermakkani, M. and Thangapandian, V. (2012). GC-MS analysis of Cassia italic leaf methanol extract. Asian Journal of Pharmaceutical and Clinical Research 5, 90-94.

Setiadi and Pariamin (2004). “Budi Daya Jeruk Asam di Kebun dan di Pot”. 128 pp. Penebar Swadaya, Jakarta.

Setiono. (2016). Produksi Benih Jeruk Bermutu. In “Makalah Kursus Teknologi Inovasi BITE”, pp. 1-12. Balai Penelitian Jeruk dan Tanaman Buah Subtropika (Balitjestro), Batu, East Java, Indonesia.

Sevvel. (2016). Types of Compound Leaves. http://www.happybotanist.com/compound-leaves [May 20, 2017].

Stojanovic, G., Palic, R., Alagic, S., and Zekovic, Z. (2000). Chemical composition and antimicrobial activity of the essential oil and CO2 extracts of semi-oriental tobacco, Otlja Flavour Fragrance Journal 15, 335-338.

Tjitrosoepomo, G. (2009). “Morfologi Tumbuhan”. UGM Press, Yogyakarta.

Uri, J., Juhasz, Z., Polgar, Z., and Banfalvi, Z. (2014). A GC-MS based metabolomics study on the tubers of commercial potato cultivars upon storage. Food Chemistry 159, 287-292.

War, A.R., Paulraj, M.G., Ahmad, T., Buhroo, A.A., Hussain, B., Ignacimuthu, S., and Sharma, H.C. (2012). Mechanisms of plant defense against insect herbivores. Plant Signaling and Behavior 7, 1306-1320.

Woyengo, T.A., Ramprasath, V.R., and Jones, P.J. (2009). Anticancer effects of phytosterols. European Journal of Clinical Nutrition 63, 813-820.

Wu, P.S., Kuo, Y.T., Chen, S.M., Li, Y., and Lou, B.S. (2014). Gas chromatography-mass spectrometry analysis of photosensitive characteristics in citrus and herb essential oils. Journal of Chromatography and Separation Techniques. 6, 1-9.

Yoshihiro, I., Akiko, S., Toshiko, H., Hajime, H., and Jingoro, S. (2004). The antibacterial effects of myrcene on staphylococcus aureus and its role in the essential oil of the tea tree (Melaleuca alternifolia). Natural Medicines 58, 10-14.

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Published

2017-06-01

How to Cite

Budiarto, R., Poerwanto, R., Santosa, E., & Efendi, D. (2017). The Potentials of Limau (Citrus amblycarpa Hassk. Ochse) as A Functional Food and Ornamental Mini Tree Based on Metabolomic and Morphological Approaches. Journal of Tropical Crop Science, 4(2), 49–57. https://doi.org/10.29244/jtcs.4.2.49-57